Can immunofluorescence on Tzanck smear be used as a substitute for direct immunofluorescence on skin biopsy in diagnosis of pemphigus disease?
Introduction: Few studies have been conducted on the use of Direct Immunofluorescence (DIF) on skin/mucosal scraping smear for diagnosis of pemphigus disease; however, the diagnostic value of DIF on the smear has not been fully evaluated.Objectives: to assess the sensitivity and specificity of DIF on skin/mucosal smear for diagnose of pemphigus in